Effect of ethanolic plant extractives on cephalic neuroendocrine system of BmNPV inoculated 5th instar larvae of Bombyx mori L. (Lepidoptera, Bombycidae)

Abstract

In this research 3000, 5000 and 8000ppm concentration of ethanolic extractives of leaves of Eupatorium odaratum, Hyptis suaveolens and fruits of Aegle marmelos were tested on mulberry silkworm fifth instar larvae of PMxCSR2 inoculated with Bombyx mori Nuclear Polyhedrosis Virus (BmNPV). Extractives of A. marmelos, H. suaveolens and E. odaratum showed promising results against BmNPV of silkworm larvae @8000 ppm. Total improvement occurs in the function of neurosecretory cells (NSC), A1 and A2 cells of median neurosecretory cells (MNC) group. Also found that, NCC I and II, corpus cardiacum (CC) lobes and fine branching of NCA I over corpus allatum (CA) showed strongly stained PF positive granules of NSM due to the subsequent treatment of given ethanolic plant extractives which reduced BmNPV infection about (30 - 40%).