Phylogeny of Indian Himalayan population of Bombus haemorrhoidalis Smith 1852 (Hymenoptera: Apidae) inferred from mitochondrial DNA sequences

Authors

  • Sunaullah Bhat
  • A. R. N. S. Subbanna
  • Amit Paschapur
  • J. Stanley
  • Sandeep Kumar
  • Jai Prakash Gupta

DOI:

https://doi.org/10.33307/entomon.v47i1.689

Abstract

Molecular variation and phylogenetic relationship of Bombus haemorrhoidalis Smith 1852 was studied using partial cytochrome oxidase I (COI) and cytochrome b (cyt b) sequences of mitochondrial genome. The COI and cyt b sequences were compared with available sequences of B. haemorrhoidalis and other Bombus species belonging to different subgenera to avail divergence within and between species. The BLASTn analysis of obtained COI sequence had cent percent identity to a hymenopteran species BOLD deposit AAC6447 (MAHYM005-10.COI-5P) which is a Bombus species from Pakistan. Both these species formed a separate cluster amongst the tested B. haemorrhoidalis species in phylogeny with pair wise genetic distance of 0.003. Moreover, the minimum evolution tree between the species revealed B. haemorrhoidalis is phylogenetically close with B. funerarius and the pair wise genetic distance between these two species was 0.102. Interestingly, B. haemorrhoidalis and B. funerarius formed separate minor cluster amongst the Bombus species tested for phylogeny. Additionally, both COI and cyt b genes were A+T biased and showed single nucleotide polymorphisms between and within species. The phylogenetic relationship of COI sequences also revealed single species status of B. haemorrhoidalis in the Indian Himalayan region and was evolutionarily associated with B. funerarius which is an another species in subgenera, Orientalibombus. The phylogeny of cyt b sequences showed that the B. haemorrhoidalis is evolutionarily close to pennsylvanicus group species. The study illustrates a complex genetic variation coupled with highly structured evolutionary divergences between and within species and provides the first report of cyt b sequence of B. haemorrhoidalis.

Author Biographies

Sunaullah Bhat

Department of Zoology, Kumaun University- SSJ Campus, Almora, Uttarakhand 263601, India

A. R. N. S. Subbanna

ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand 263601, India

Amit Paschapur

ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand 263601, India

J. Stanley

ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand 263601, India.

Sandeep Kumar

Department of Zoology, Kumaun University- SSJ Campus, Almora, Uttarakhand 263601, India

Jai Prakash Gupta

ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand 263601, India.

Downloads

Published

2022-03-31

How to Cite

Bhat, S. ., Subbanna, A. R. N. S. ., Paschapur, A. ., Stanley, J. ., Kumar, S. ., & Gupta, J. P. . (2022). Phylogeny of Indian Himalayan population of Bombus haemorrhoidalis Smith 1852 (Hymenoptera: Apidae) inferred from mitochondrial DNA sequences. ENTOMON, 47(1), 61–70. https://doi.org/10.33307/entomon.v47i1.689

Issue

Section

Articles